Influence of Er,Cr:Ysgg Associated or not To 5% Fluoride Varnish in the Erosion Treatment of Bovine Enamel
DOI:
https://doi.org/10.17921/2447-8938.2019v21n4p365-70Resumo
Abstract
The objective of this in vitro study was to evaluate the influence of the Er,Cr:YSGG laser associated or not to a desensitizing agent in the treatment of erosive lesions. Forty specimens with dimensions of 4mm x 4mm and 3mm thickness were divided into 4 groups (n = 10): G1- no treatment; G2- 5% fluoride varnish; G3- Er,Cr: YSGG; G4 - fluoride varnish + laser. The specimens were immersed in erosive drink 3 times a day for 1 minute with an average interval of 2 hours between cycles for a period of 10 days. The treatments were performed according to the groups and the surface roughness and the wear profile were analyzed by scanning confocal microscopy. The normality (Kolmogorov-Smirnov) and homogeneity (Levene's) of the tests were evaluated. After these analyzes, the surface roughness data were submitted to the statistical analysis of Variance Analysis (ANOVA). All tests adopted a significance level of 5% (α = 0.05). At the representative images of the wear profile, the morphology of bovine dental enamel in its control and eroded areas were compared and qualitatively discussed. As regard surface roughness, there was no statistically significant difference between the groups. The qualitative analysis of the loss of volume showed that all experimental groups showed significant superficial morphology differences. Considering the limitations of an in vitro study, it can be concluded that the treatments performed were not able to treat dental erosion satisfactorily, indicating the need for more treatment sessions.
Keywords: Tooth Erosion. Dental Enamel. Fluoride. YSGG Laser.
Resumo
O objetivo desse estudo in vitro foi avaliar a influência do laser Er,Cr:YSGG associado ou não a um agente dessensibilizante no tratamento de lesões erosivas. Foram confeccionados 40 espécimes com dimensões de 4mm x 4mm e 3mm de espessura, divididos em 4 grupos (n=10): G1- nenhum tratamento; G2- verniz fluoretado 5%; G3- aplicação do laser Er,Cr:YSGG; G4- verniz fluoretado + laser. Os espécimes foram imersos em bebida erosiva, 3 vezes por dia, durante 1 minuto, com intervalo médio de 2 horas entre os ciclos, por um período de 10 dias. Os tratamentos foram realizados de acordo com os grupos e que foram analisados a rugosidade superficial e o perfil de desgaste por meio de microscopia confocal de varredura. Avaliou-se a normalidade (Kolmogorov-Smirnov) e homogeneidade (Levene’s) dos dados. Após estas análises, os dados de rugosidade superficial foram submetidos ao teste estatístico de Análise de Variância (ANOVA). Todos os testes adotaram nível de significância de 5% (α=0,05). Através da obtenção das imagens representativas do perfil desgaste, a morfologia do esmalte dental bovino em suas áreas controle e erodida foram comparadas e qualitativamente discutidas. Quanto à rugosidade superficial, não houve diferença estatisticamente significativa entre os grupos. A análise qualitativa da perda de volume mostrou que todos os grupos experimentais apresentaram diferenças significativas na morfologia superficial. Considerando as limitações de um estudo in vitro pode-se concluir que os tratamentos realizados não foram capazes de tratar a erosão dentária de forma satisfatória, indicando a necessidade de mais sessões de tratamento.
Palavras-chave: Erosão dentária. Esmalte dental. Flúor. Laser de YSGG.
Referências
West NX, Lussi A, Seong J, Hellwig E. Dentin hypersensitivity: pain mechanisms and aetiology of exposed cervical dentin. Clin Oral Invest 2012;17:S1-S19. 10.1007/s00784-012-0887-x.
Dias, ARC et al. Tratamento de lesões cervicais. In: Pereira JC, Anauate-Netto, c, Gonçalves Aa. Dentística: Uma abordagem multidisciplinar. São Paulo: Artes Médicas; 2014.
Colombo M, Mirando M, Rattalino D, Beltrami R, Chiesa M, Poggio C. Remineralizing effect of a zinc-hydroxyapatite toothpaste on enamel erosion caused by soft drinks: Ultrastructural analysis. J ClinExp Dent 2017;9:e861-868 doi 10.4317/jced.53790.
Ostrowska A, Szymanski W, Kolodziejczyk L, Rzepkowska EB.Evaluation of the Erosive Potential of Selected Isotonic Drinks: In Vitro Studies. Adv Clin Exp Med 2016; 25:1313-1319 doi <http://dx.doi.org/10.17219/acem/ 62323.
Farag ZHA, Awooda EM. Dental erosion and dentin hypersensitivity among adult asthmatics and non-asthmatics hospital-based: a preliminary study. Open Dent J 2016;10:587-93 doi 10.2174/1874210601610010587.
Comar LP, Cardoso CA, Charone S, Grizzo LT, Buzalaf MA, Magalhães AC. TiF4 and NaF varnishes as anti-erosive agents on enamel and dentin erosion progression in vitro. J Appl Oral Sci 2015; 23:14-8 doi dx.doi.org/10.1590/1678-775720140124.
Lammers PC, Borggreven JM, Driessens FC. Influence of fluoride and pH on in vitro remineralization of bovine enamel.Caries Res 1992;26(1):8-13 doi 10.1159/000261418.
Buzalaf MAR, Kobayashi CAN, Tucunduva, S. Histórico do uso de fluoretos em saúde bucal. In: Buzalaf, MAR. Fluoretos e saúde bucal. São Paulo: Livraria Santos; 2008.
Alves RX, Fernandes GF, Razzolini MTP, Frazão P, Marques RAA, Narval PC. Evolução do acesso à água fluoretada no Estado de São Paulo, Brasil: dos anos 1950 à primeira década do século XXI. Cad. Saúde Pública 2012 doi dx.doi.org/10.1590/S0102- 311X2012001300008.
Oliveira RM, Souza VM, Esteves CM, de Oliveira Lima-Arsati YB, Cassoni A, Rodrigues JA, Brugnera Junior A. Er,Cr:YSGG Laser Energy delivery: pulse and power effects on enamel surface and erosive resistance. Photomed Laser Surg 2017;35(11):639-646. doi: 10.1089/pho.2017.4347.
Fujii M, Kitasako Y, Sadr A, Tagami J. Roughness and pH changes of enamel surface induced by soft drinks in vitro applications of stylus profilometry, focus variation 3D scanning microscopy and micro pH sensor. Dent Mater J 2011;30:404–410 doi http://dx.doi.org/10.4012/dmj.2010-204.
Tanaka JLO, Medici Filho E, Salgado JA,Salgado MAC, Moraes LC,Moraes MEL et al. Comparative analysis of human and bovine teeth: radiographic density. Braz Oral Res 2008;22(4):346-51 doi: 10.1590/S1806-83242008000400011.
Wegehaupt F, Gries D, Wiegand A, Attin T.Is bovine dentine an appropriate substitute for human dentine in erosion/abrasion tests?. J Oral Rehabil 2008;35:390-39 doi: 10.1111/j.1365-2842.2007.01843. x.
Lussi A, Megert B, Shellis RP, Wang X. Analysis of the erosive effect of different dietary substances andmedications. Bras J Nutr 2012;107(2):252-262 doi: 10.1017/S0007114511002820, 2012.
Alexandria AK, Vieira TI, Pithon MM, da Silva Fidalgo TK, Fonseca-Gonçalves A, Valença AM, et al. In vitro enamel erosion and abrasion-inhibiting effect of different fluoride varnishes. Arch Oral Biol 2017;77:39-43. doi: 10.1016/j.archoralbio.2017.01.010.
Moynihan PJ. The role of diet and nutrition in the etiology and prevention of oral diseases. Bulletin of the World Health Organization. 2005;83:694-9.
Wiegand A, Magalhães AC, Attin T: Is titanium tetrafluoride (TiF4) effective to prevent carious and erosive lesions? A review of the literature. Oral Health Prev Dent 2010;8:159-64 doi http://dx.doi.org/10.1016/j.
Scaramucci T, Borges AB, Lippert F, Frank NE, Hara AT: Sodium fluoride effect on erosion-abrasion under hyposalivatory simulating conditions. Arch Oral Biol 2013;58:1457-63 doi http://dx.doi.org/10.1016/j. Archoralbio.2013.06.004.
Scaramucci T, Borges AB, Lippert F, Zero DT, Aoki IV, Hara AT: Anti-erosive properties of solutions containing fluoride and different film-forming agents. J Dent 2015;43:458-65 doi doi.org/10.1159/000443619.
Magalhães AC, Wiegand A, Rios D, Buzalaf MA, Lussi A. Fluoride in dental erosion. Monogr Oral Sci 2011;22:158-70 doi doi.org/10.1159/000325167.
Van den berghe, L,De boever, J,Adriaens, PA. Hyperesthésie du collet: ontogenèse etthérapie. Un status questionis. Rev Belge Med Dent 1984;39(1):2-6.
Camilotti V, Zilly J, Busato Pdo M, Nassar CA, Nassar PO. Desensitizing treatments for dentin hypersensitivity: a randomized, split-mouth clinical trial. Braz Oral Res 2012;26:263-8 doi dx.doi.org/10.1590/S1806-83242012000300013.
Huysmans MC, Young A, Ganss C. The role of fluoride in erosion therapy. In: Lussi A, Ganss C. Erosive Tooth wear: from diagnosis to therapy. Monogr Oral Sci. Basel 2014;25:230-43 doi dx.doi.org/10.1159/000360555.
Sar Sancakli H, Austin RS, Al-Saqabi F, Moazzez R, Bartlett D. The influence of varnish and high fluoride on erosion and abrasion in a laboratory investigation. Aust Dent J 2015;60(1):38-42 doi: 10.1111/adj.12271.
Gaffar A. Treating hypersensitivity with fluoride varnishes. Compend Contin Educ Dent 1998; 19:1088-90.
Assis JS, Rodrigues LK, Fonteles CS, Colares RC, Souza AM,Santiago SL. Dentin hypersensitivity after treatment with desensitizing agents: a randomized, double-blind, split-mouth clinical trial. Braz Dent J 2011;22:157-16 doi /dx.doi.org/10.1590/S0103-64402011000200012.
Stead WJ, Orchardson R; Warren B. A mathematical model of potassium ion diffusion in dentinal tubules. Arch Oral Biol 1996;41(7): 679-87 doi.org/10.1016/S0003-9969(96)00073-8.
Sakae LO, Bezerra SJC, João-Souza SH, Borges AB, Aoki IV.An in vitro study on the influence of viscosity and frequency of application of fluoride/tin solutions on the progression of erosion of bovine enamel.Arch Oral Biol 2018;89:26-30 doi 10.1016/j.archoralbio.2018.01.017.
Aranha AC, Pimenta LA,Marchi GM. Clinical evaluation of de- sensitizing treatments for cervical dentin hypersensitivity. Braz Oral Res 2009;23(3):333-9 /dx.doi.org/10.1590/S1806-83242009000300018.
Dos Reis Derceli, J et al. Effect of pretreatment with an Er:YAG laser and fluoride on the prevention of dental enamel erosion. Lasers Med Sci 2015;30(2):857-62. doi: 10.1007/s10103-013-1463-6.
Moslemi M, FekrazaD R, Tadayon N, Ghorbani M, Torabzadeh H, Shadkar MM. Effects of ER,Cr:YSGG laser irradiation and fluoride treatment on acid resistance of the enamel.Pediatr Dent 2009.31(5):409-13.
Freitas PM, Raposo - HiloM, Eduardo CP, Featherstone JD. In vitro evaluation of erbium,chromium:yttrium-scandium-gallium-garnet laser-treated enamel demineralization. Lasers Med Sci 2010;25(2):165-70. doi: 10.1007/s10103-008-05974.
Schwarz F.Desensitizing effects of an Er:YAG laser on hypersensitive dentine. A controlled, prospective clinical study. J Clin Periodontol 2002;29 (3):211-5.211- 5.
Ehlers V, Ernst CP, Reich M, Kämmerer P, Willershausen B. Clinical comparison of gluma and Er:YAG laser treatment of cervically exposed hypersensitive dentin. Am J Dent 2012;25(3):131-5
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