In vitro Evaluation of the Antimicrobial Potential of Salvia officinalis L. against Oral Pathogens
DOI:
https://doi.org/10.17921/2447-8938.2019v21n2p129-133Resumo
Abstract
The emergence of multidrug-resistant strains to antibiotics has motivated the search for new substances with antimicrobial activity, especially those derived from medicinal plants. Salvia officinalis L. is a medicinal plant that arouses scientific interest due to being associated with multiple therapeutic effects. The purpose of this study was to evaluate the in vitro antimicrobial potential of S. officinalis L. against pathogens in the oral cavity. The antimicrobial potential of the ethanol extract of leaf of S. officinalis L was evaluated by broth microdilution, with determination of minimum inhibitory concentration (MIC) and Minimum bactericidal/Fungicide concentration (MBC / MFC), against the species Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguis, Candida albicans, Candida glabrata, Candida guillermond, Candida krusei and Candida tropicalis. The extract showed moderate antifungal potential before Candida species (MIC = 1 mg/mL). And for the species of Streptococcus, the antimicrobial activity was from moderate to strong whose MIC ranged from 0.25 to 1 mg/mL. In this study, the extract from the leaves of S. officinalis L. presented oral cavity antimicrobial activity against pathogens. These results point to S. officinalis as a possible source of active ingredients in the development of formulations with antimicrobial activity of dental use.
Keywords: Plants, Medicinal. Salvia officinalis L. Products with Antimicrobial action.
Resumo
O surgimento de cepas multirresistentes a antibióticos tem motivado a busca de novas substâncias com atividade antimicrobiana, especialmente aquelas oriundas de plantas medicinais. A Salvia officinalis L. é uma planta medicinal que desperta interesse científico por estar associada a múltiplos efeitos terapêuticos. O objetivo do presente estudo foi avaliar o potencial antimicrobiano in vitro da S. officinalis L. frente a patógenos da cavidade bucal. O potencial antimicrobiano do extrato etanólico da folha da S. officinalis L foi avaliado por meio da microdiluição em caldo, com determinação da Concentração Inibitória Mínima (CIM) e Concentração Bactericida/Fungicida Mínima (CBM/CFM), frente as espécies Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguis, Candida albicans, Candida glabrata, Candida guillermond, Candida krusei e Candida tropicalis. O extrato apresentou moderado potencial antifúngico frente às espécies de Candida (CIM = 1 mg/mL). Para as espécies de Streptococcus, o potencial antimicrobiano foi considerado forte a moderado, com valores de CIM variando entre 0,25 a 1 mg/mL. Neste estudo, o extrato da folha de S. officinalis L. apresentou potencial antimicrobiano contra patógenos da cavidade bucal. Esses resultados apontam a S. officinalis como uma possível fonte de princípios ativos no desenvolvimento de formulações com atividade antimicrobiana de uso odontológico.
Palavras-chave: Plantas Medicinais. Salvia officinalis L. Produtos com Ação Antimicrobiana.
Referências
Silva MSPS, Brandão DO, Chaves TP, Formiga Filho ALN, Costa EMMB, Santos VL, et al. Study bioprospecting of medicinal plant extracts of the semiarid northeast: contribution to the control of oral microorganisms. Evid Based Complement Alternat Med 2012; 2012.
Melo AFM, Santos EJV, Souza LFC, Carvalho AAT, Pereira MSV, Higino JS. In vitro antimicrobial activity of an extract of Anacardium occidentale L. against Streptococcus species. Bras J Pharmacogn 2006;16(2):202-5.
Lins R, Vasconcelos FHP, Leite RB, Coelhos-Soares RS, Barbosa DN. Clinical evaluation of mouthwash with extracts from aroeira (Schinus terebinthifolius) and chamomile (Matricaria recutita L.) on plaque and gingivitis. Rev Bras Plantas Med 2013;15(1):112-20.
Kurek A, Nadkowska P, Pliszka S, Wolska KI. Modulation of antibiotic resistance in bacterial pathogens by oleanolic acid andursolicacid. Phytomedicine 2012;19(6)515-9.
Freires IA, Murata RM, Furletti VF, Sartoratto A, Alencar SM, Figueira GM, et al. Coriandrum sativum L. (Coriander) Essential oil: antifungal activity and mode of action on Candida spp., and molecular targets affected in human whole-genome expression. Plos One 2014;9(6):e99086. doi: https://doi.org/10.1371/journal.pone.0099086
Lima RF, Alves EP, Rosalen PL, Ruiz ALTG, Duarte MCT, Góes VFF, et al. Antimicrobial and Antiproliferative Potential of Anadenanthera colubrina (Vell.) Brenan. Evid Based Complement Alternat Med 2014. doi: http://dx.doi.org/10.1155/2014/802696
Freires IA, Bueno-Silva B, Galvão LCC, Duarte MCT, Sartoratto A, Figueira GM, et al. The effect of essential oils and bioactive fractions on streptococcus mutans and Candida albicans Biofilms: a confocal analysis. Evid Based Complement Alternat Med 2015; 2015. doi: http://dx.doi.org/10.1155/2015/871316
Ferreira GLS, Péres ALAL, Rocha IM, Pinheiro MA, Castro RD, Carlo HL, et al. Does scientific evidence for the use of natural products in the treatment of oral candidiasis exist? a systematic review. Evid Based Complement Alternat Med 2015; 2015. doi: http://dx.doi.org/10.1155/2015/871316
Garcia CS, Menti C, Lambert AP, Barcellos T, Moura S, Calloni C, et al. Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells. An Acad Bras Cienc 2016;88(1):281-92. doi: 10.1590/0001-3765201520150344.
Ghorbanpour M, Hatami M, Kariman K, Abbaszadeh Dahaji P. Phytochemical variations and enhanced efficiency of antioxidant and antimicrobial ingredients in Salvia officinalis as Inoculated with Different Rhizobacteria. Chem Biodivers 2016;13(3):319-30. doi: 10.1002/cbdv.201500082.
Garcia CSC, Lambert APF, Henriques JAP, Ely MR. In vitro evaluation of the biological potential of Salvia officinalis L. Sci Med 2012;22(3):131-7.
Rodrigues MR, Kanazawa LK, das Neves TL, da Silva CF, Horst H, Pizzolatti MG, et al. Antinociceptive and anti-inflammatory potential of extract and isolated compounds from the leaves of Salvia officinalis in mice. J Ethnopharmacol 2012;139(2):519-26. doi: 10.1016/j.jep.2011.11.042.
Abu-Darwish MS, Cabral C, Ferreira IV, Gonçalves MJ, Cavaleiro C, Cruz MT, et al. Essential oil of common sage (Salvia officinalis L.) from Jordan: Assessment of safety in mammalian cells and its antifungal and anti-inflammatory potential. Biomed Res Int 2013;2013. doi: 10.1155/2013/538940
Akaberi M, Mehri S, Iranshahi M. Multiple pro-apoptotic targets of abietane diterpenoids from Salvia species. Fitoterapia 2015;100:118-32. doi: 10.1016/j.fitote.2014.11.008.
Badiee P., Nasirzadeh AR, Motaffaf M. Comparison of Salvia officinalis L. essential oil and antifungal agents against Candida species. J Pharm Technol Drug Res 2012;1(7):1-5.
Kontogianni VG, Tomic G, Nikolic I, Nerantzaki AA, Sayyad N, Stosic-Grujicic S, et al. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity. Food Chem 2013;136(1):120-9.
Mossi AJ, Cansian RL, Paroul N, Toniazzo G, Oliveira JV, Pierozan MK, et al. Morphological characterisation and agronomical parameters of different species of Salvia sp. (Lamiaceae). Braz J Biol 2011;71(1):121-9.
Horiuchi K, Shiota S, Kuroda T, Hatano T, Yoshida T, Tsuchiya T. Potentiation of antimicrobial activity of aminoglycosides by carnosol from Salvia officinalis. Biol Pharm Bul 2007;30(2):287-90.
Pierozan MK, Pauletti GF, Rota L, Santos ACA, Lerin LA, di Luccio M, et al. Chemical characterization and antimicrobial activity of essential oils of Salvia L. species. Ciênc Tecnol Aliment 2009; 29(4):764-70.
Alizadeh A, Shaabani M. Essential oil composition, phenolic content, antioxidant and antimicrobial activity in Salvia officinalis L. cultivated in Iran. Adv Space Biol Med 2012;6(1):221-6.
Porte A, Godoy RLO, Mais-Porte LH. Chemical composition of sage (Salvia officinalis L.) essential oil from de Rio de Janeiro state (Brazil). Rev Bras Plantas Med 2013;13(3):438-41.
Oliveira FQ, Gobira B, Guimarães C, Batista J, Barreto M, Souza M. Espécies vegetais indicadas na odontologia. Bras J Pharmacogn 2007;17(3):466-76.
Clinical and Laboratory Standards Institute (CLSI). Protocol M27-A3. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Pennsylvania: NCCLS; 2008.
Clinical and Laboratory Standards Institute (CLSI). Protocol M07-A8. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Pennsylvania: NCCLS; 2009.
Khalil R, Li Z. Antimicrobial activity of essential oil of Salvia officinalis L. collected in Syria. Biotechnol 2011;10(42):8397-402.
Moreira MR, Souza AB, Moreira MA, Bianchi TC, Carneiro LJ, Estrela FT, et al. RP-HPLC analysis of manool-rich Salvia officinalis extract and its antimicrobial activity against bacteria associated with dental caries. Bras J Pharmacogn 2013;23(6):870-6.
Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou IB. Composition and antimicrobial activity of the essential oils of two Origanum species. J Agric Food Chem 2001;49(9):4168-70.
Sookto T, Srithavaj T, Thaweboon S, Thaweboon B, Shrestha B. In vitro effects of Salvia officinalis L. essential oil on Candida albicans. Asian Pac J Trop Biomed 2013;3(5):376-80.
Vieira DRP, Amaral FMM, Maciel MCG, Nascimento FRF, Libério AS. Plants and chemical constituents used in dentistry: review of ethnopharmacological and antimicrobial activity studies in oral pathogens. Rev Bras Pl Med 2014;16(1):135-67. doi: http://dx.doi.org/10.1590/S1516-05722014000100020
Nogueira MA, Diaz G, Tagami PM, Lorschiede J. Antimicrobial activity of essential oils and propolis against oral pathogens. Rev Ciênc Farm Apl 2007;28(1):93-7.
Babpour E, Angaji SA, Angaji SM. Antimicrobial effects of four medicinal plants on dental plaque. J Med Plant Res 2009;3(3):132-7.
Pereira JV, Pereira MSV, Sampaio FC, Sampaio MCC, Alves PM, Araújo CRF, et al. In vitro antibacterial and antiadherence effect of the extract of the Punica granatum Linn. upon dental biofilm microrganisms. Bras J Pharmacogn 2006;16(1):88-93.
Diniz DN, Macêdo-Costa MR, Pereira MSV, Pereira JV, Higino JS. In vitro antifungal effect of leaves and bark of Myrciaria cauliflora Berg. extracts upon oral microorganisms. Rev odontol UNESP 2010;39(3):151-6.
Lawrence JR, Zhu B, Swerhone GD, Topp E, Roy J, Wassenaar LI, et al. Community-Level Assessment of the Effects of the Broad-Spectrum Antimicrobial Chlorhexidine on the Outcome of River Microbial Biofilm Development. Appl Environ Microbiol 2008;74(11):3541-50.
Rocha EALSS, Medeiros ACD, Carvalho AVOR, Andrade SRA, Trovão DMMB, Costa EMMB. In vitro antimicrobial activity os hydroalcoholic extracts from brazilian northeastern medicinal plants againts Ctreptococcus. Pesq Bras Odontopediatr Clin Integr 2013;13(2):233-8. doi: 10.4034/PBOCI.2013.133.02
Marinho BVS, Araújo ACS. Uso dos enxaguatórios bucais sobre a gengivite e biofilme dental. Int J Dent 2007;6(4):124-31.
Corrêa EMC, Andrade ED. Dental management of HIV/AIDS patients. Rev Odonto Ciênc 2005;20(49):281-9.
Ten Cate JM, Klis FM, Pereira-Cenci T, Crielaard W, de Groot PW. Molecular and cellular mechanisms that lead to Candida biofilm formation. J Dent Res 2009;88(2):105-15.
Cavalcanti AL, Almeida LFD, Sá SCVL, Silva KF, Meneses RO, Castro RD. In vitro antifungal activity of mouthwashes against Candida spp. Rev Odontol UNESP 2009;38(5):313-7.
Pérez ALAL, Cardoso AMR, Cavalcanti YW, Almeida LFD, Padilha WN. Antifungal Activity of Mouthwashes on Candida spp. Rev Bras Cienc Saúde 2011;15(1):69-74.
Lubian CT, Teixeira JM, Lund RG, Nascente OS, Del pino FAB. Antifungal activity of the aqueous extract from Arctium minus (Hill) Bernh. (Asteraceae) on oral Candida species. Rev Bras Plantas Med 2010;12(2):157-62.
Alves TMS, Silva CA, Silva NB, Valença AMG, Medeiros EB. Antimicrobial activity of fluoridated products on biofilm-forming bactéria: an in vitro study. Pesq Bras Odontoped Clin Integ 2010;10(2):209-16.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Os autores devem ceder expressamente os direitos autorais à Kroton Educacional, sendo que a cessão passa a valer a partir da submissão do artigo, ou trabalho em forma similar, ao sistema eletrônico de publicações institucionais. A revista se reserva o direito de efetuar, nos originais, alterações de ordem normativa, ortográfica e gramatical, com vistas a manter o padrão culto da língua, respeitando, porém, o estilo dos autores. As provas finais serão enviadas aos autores. Os trabalhos publicados passam a ser propriedade da Kroton Educacional, ficando sua reimpressão total ou parcial, sujeita à autorização expressa da direção da Kroton Educacional. O conteúdo relatado e as opiniões emitidas pelos autores dos artigos são de sua exclusiva responsabilidade.
